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Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Fast flexible multiphoton fluorescence lifetime imaging using acousto-optic deflector.

Jing Qi¹, Yonghong Shao, Lixin Liu

¹Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China.

|August 14, 2013

Summary

We developed a fast fluorescence lifetime imaging microscopy system. It enables rapid, flexible imaging of specific sample areas, ideal for discontinuous regions of interest.

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Area of Science:

Biophotonics and advanced microscopy techniques.

Background:

Fluorescence lifetime imaging microscopy (FLIM) is crucial for biological research.
Current FLIM systems can be limited in speed and flexibility for imaging specific regions.

Purpose of the Study:

To introduce a novel, fast, and flexible fluorescence lifetime imaging microscopy (FLIM) system.
To demonstrate the system's capability for addressable measurements and imaging of discontinuous regions of interest (ROI).

Main Methods:

Utilized a two-dimensional acousto-optic deflector for rapid beam scanning.
Implemented random access to regions of interest (ROI) for targeted imaging.
Tested with standard fluorescent dyes and biological samples.

Main Results:

Achieved fast and flexible fluorescence lifetime measurements.
Demonstrated addressable measurements, allowing targeted data acquisition.
Successfully performed FLIM on discontinuous regions of interest within samples.

Conclusions:

The developed FLIM system offers significant improvements in speed and flexibility.
The system is particularly advantageous for imaging complex or discontinuous biological structures.
This technology enables more efficient and targeted fluorescence lifetime analysis.