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Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope
Charles-Edouard Leroux1, Alexei Grichine, Irène Wang
1University of Grenoble 1/CNRS, LIPhy UMR 5588, Grenoble, France.
Optics Letters
|August 14, 2013
Summary
Optical aberrations in living cells significantly impact fluorescence fluctuations microscopy (FFM). Adaptive optics (AO) corrects these aberrations, enabling clearer FFM measurements through single cells and paving the way for complex 3D imaging.
Area of Science:
- Biophysics
- Optical Microscopy
- Cell Biology
Background:
- Fluorescence fluctuations microscopy (FFM) is sensitive to optical aberrations.
- Living cells introduce optical aberrations when focusing light.
- Accurate FFM requires correction of these aberrations.
Purpose of the Study:
- To investigate the effect of optical aberrations on FFM.
- To demonstrate adaptive optics (AO) for aberration correction in FFM.
- To enable FFM in complex biological samples.
Main Methods:
- Performing FFM measurements in an aqueous fluorescent solution.
- Introducing optical aberrations using a single living cell.
- Implementing an adaptive optics (AO) system to correct aberrations.
Main Results:
- FFM is highly sensitive to cell-induced optical aberrations.
- AO effectively removes aberration-related bias in FFM data.
- AO enhances FFM performance through single cellular layers.
Conclusions:
- AO is crucial for accurate FFM through biological samples.
- This technique advances FFM for complex multicellular imaging.
- FFM with AO opens new avenues for studying 3D biological systems.
