Evanescent-wave-induced frequency shift for optical superresolution imaging
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Updated: May 8, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
Published on: January 6, 2026
Xiang Hao1, Cuifang Kuang, Yanghui Li
1State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou, China.
Abstract:
We propose a method to enhance the resolution and break the diffraction limit. The superresolution imaging is realized by incorporating total internal reflection (TIR) illumination with a passive spatial frequency shift mechanism. Meanwhile, TIR supplies a surface field with a limited penetration depth, which demonstrates that the axial resolution can be improved simultaneously. The superresolution capability is confirmed both theoretically and experimentally. Compared with microfiber-based former work, this idea possesses promising merits, providing a wider viewing field and a simpler configuration for variable illumination orientations, thereby implying abundant application potentials.
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