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06:03
Isolation and Characterization of Microvesicles from Peripheral Blood
Published on: January 6, 2017
C-src enriched serum microvesicles are generated in malignant plasma cell dyscrasia.
Giuseppe Di Noto1, Lucia Paolini, Andrea Zendrini
1Department of Molecular and Translational Medicine, Faculty of Medicine, University of Brescia, Brescia, Italy.
Plos One
|August 14, 2013
Summary
Monoclonal immunoglobulin Free Light Chains (FLCs) from malignant cells are transported in specific vesicles, suggesting a novel mechanism in plasma cell dyscrasias like Multiple Myeloma.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- Plasma cell dyscrasias, including Multiple Myeloma (MM), are immunosecretory disorders.
- Monoclonal immunoglobulin Free Light Chains (FLCs) are biomarkers in these conditions.
- The biological behavior of FLCs in malignant versus benign conditions is not well understood.
Purpose of the Study:
- To investigate the biological differences and transport mechanisms of monoclonal FLCs.
- To characterize the role of microvesicles and exosomes in FLC dissemination.
- To explore potential mechanisms of disease progression in plasma cell dyscrasias.
Main Methods:
- Utilized endothelial and heart muscle cell lines to study FLC internalization.
- Analyzed FLC transport via microvesicles and exosomes.
- Investigated the presence of specific markers (Hsp70, annexin V, c-src) in vesicles from patients with MM, AL Amyloidosis, and MGUS.
Main Results:
- Endothelial and heart muscle cells internalize both kappa and lambda FLCs.
- Internalized FLCs are rerouted extracellularly via microvesicles and exosomes, with potential for re-uptake by adjacent cells.
- FLCs from malignant B lymphocytes are specifically packaged in vesicles containing Hsp70, annexin V, and c-src.
- Increased microvesicle and exosome production observed in MM and AL Amyloidosis patients.
- Serum vesicles from MM, AL Amyloidosis, and MGUS patients contain FLCs.
- Vesicles from MM and AL Amyloidosis patients show significantly higher positivity for Hsp70, annexin V, and c-src compared to MGUS and controls.
Conclusions:
- FLCs can be transported via microvesicles in the bloodstream.
- This suggests a novel mechanism for FLC dissemination and potential c-src activation in plasma cell dyscrasias.
- Characterization of FLC-containing vesicles may offer new diagnostic or therapeutic targets.
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