Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cognitive engagement induces area-specific fingerprints of dopamine, acetylcholine, serotonin, glutamate and GABA in prefrontal cortex and striatum.

bioRxiv : the preprint server for biology·2026
Same author

Nonexhaustive microextraction as a step toward more sustainable chemical analysis in the field and the clinic.

Nature protocols·2026
Same author

<i>In Vivo</i> Negligible Depletion SPME for the Determination of Free and Total Concentrations of Anandamide and 2-Arachidonoylglycerol in the Brain of a Parkinson's Disease Rat Model.

Analytical chemistry·2026
Same author

Defects in auxiliary fuel oxidation and mitochondrial pyruvate transport mark transition to overt heart failure in Tgαq*44 mice.

Journal of translational medicine·2026
Same author

High-throughput screening of per- and polyfluoroalkyl substances in human plasma using biocompatible solid-phase microextraction coupled with mass spectrometry via microfluidic open interface.

Analytica chimica acta·2026
Same author

Comprehensive analysis of exhaled breath VOCs using GC-MS and GC×GC-TOF-MS: a comparative platform evaluation with TFME and NTD sampling for free and total concentrations.

Analytical and bioanalytical chemistry·2025

Related Experiment Video

Updated: May 8, 2026

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool
05:31

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool

Published on: May 25, 2021

Determination of polycyclic aromatic hydrocarbons in solid matrices using automated cold fiber headspace solid phase

Jun Guo1, Ruifen Jiang, Janusz Pawliszyn

  • 1Department of Chemistry, University of Waterloo, Ontario, N2L 3G1, Canada.

Journal of Chromatography. A
|August 15, 2013
PubMed
Summary

This study optimized cold fiber headspace solid-phase microextraction (CF-HS-SPME) for polycyclic aromatic hydrocarbons (PAHs). Programmed coating temperature and diethylamine enhanced extraction efficiency, achieving over 90% recovery for many PAHs.

Keywords:
Cold fiberPolycyclic aromatic hydrocarbons (PAHs)Sample preparationSoil analysisSolid phase microextraction (SPME)

More Related Videos

A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter
04:39

A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter

Published on: June 13, 2025

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Related Experiment Videos

Last Updated: May 8, 2026

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool
05:31

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool

Published on: May 25, 2021

A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter
04:39

A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter

Published on: June 13, 2025

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Area of Science:

  • Analytical Chemistry
  • Environmental Science

Background:

  • Polycyclic Aromatic Hydrocarbons (PAHs) are persistent organic pollutants requiring efficient extraction methods.
  • Traditional methods for PAH extraction from solid matrices can be time-consuming and solvent-intensive.

Purpose of the Study:

  • To investigate and optimize the extraction efficiency of a cold fiber headspace solid-phase microextraction (CF-HS-SPME) system for PAHs.
  • To develop a high-throughput, solventless sample preparation method for environmental analysis.

Main Methods:

  • Theoretical and experimental investigation of CF-HS-SPME system parameters.
  • Optimization of programmed coating temperature for enhanced analyte desorption.
  • Evaluation of organic solvents, including diethylamine, to improve extraction recovery.

Main Results:

  • Programmed coating temperature method significantly improved extraction efficiency for most PAHs compared to traditional approaches.
  • Recoveries exceeded 90% for the majority of tested compounds from a sand matrix, indicating exhaustive extraction.
  • Diethylamine facilitated exhaustive extraction of volatile compounds and enhanced recoveries of 60-75% for semi-volatile PAHs.

Conclusions:

  • The automated CF-HS-SPME system with programmed coating temperature offers a convenient and efficient approach for PAH extraction.
  • This method facilitates high-throughput and solventless sample preparation, suitable for environmental monitoring.
  • The system demonstrates high extraction efficiency and recovery rates, making it a valuable tool for quantitative PAH analysis.