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Updated: May 8, 2026

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples
Published on: March 28, 2015
Occurrence of Human Cosavirus in Wastewater and River Water in Japan
Eiji Haramoto1, Mikie Otagiri2
1International Research Center for River Basin Environment, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511, Japan. eharamoto@yamanashi.ac.jp.
Abstract:
Human cosavirus was detected using reverse transcription-quantitative polymerase chain reaction in 71 % of raw sewage and 29 % each of secondary-treated sewage and river water samples in the Kofu Basin, Japan. The highest concentration was 2.80 × 10(6) copies/l. Nucleotide sequence analysis revealed multiple genotypes of the virus in wastewater.
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