Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 8, 2026

Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model
12:23

Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model

Published on: April 24, 2020

Normal sequential changes in neutrophil CD64 expression after total joint arthroplasty.

Narutaka Katoh1, Jinju Nishino, Keita Nishimura

  • 1Department of Orthopaedic Surgery, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo, Japan, narubi@med.teikyo-u.ac.jp.

Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association
|August 15, 2013
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optical Coherence Tomography Characterization of Coronary Lithotripsy Following Atherectomy for Treatment of Severely Calcified Lesions.

Circulation. Cardiovascular interventions·2026
Same author

Paclitaxel drug-coated balloon angioplasty for de novo coronary lesions in an expanded real world clinical setting: the multicenter ALLIANCE registry.

Cardiovascular intervention and therapeutics·2026
Same author

Dual-Prep registry: Atherectomy devices and intravascUlAr lithotripsy for the PREParation of heavily calcified coronary lesions registry, 1-year results.

Cardiovascular intervention and therapeutics·2026
Same author

Clinical expert consensus document on rotational atherectomy from the Japanese association of cardiovascular intervention and therapeutics: update 2026.

Cardiovascular intervention and therapeutics·2026
Same author

Localization of unstable intramural ventricular tachycardia circuit using live directional activation mapping.

HeartRhythm case reports·2025
Same author

Case Report: Overcoming calcified nodules by repeated aggressive debulking and stent-free percunatenous coronary intervention using a drug-coated balloon.

Frontiers in cardiovascular medicine·2025

Neutrophil CD64 expression significantly increases after joint replacement surgery, peaking around day 3 and returning to baseline by day 5. This pattern helps in diagnosing post-operative infections.

Area of Science:

  • Biomedical Engineering
  • Immunology
  • Orthopedic Surgery

Background:

  • Neutrophil CD64 is a sensitive marker for infection.
  • Accurate interpretation of CD64 requires understanding its normal changes after surgery.
  • Post-operative kinetics of CD64 after total joint arthroplasty were previously unclear.

Purpose of the Study:

  • To clarify the normal sequential changes in neutrophil CD64 expression after total joint arthroplasty.
  • To establish a baseline for interpreting CD64 levels in the early post-operative period.

Main Methods:

  • Quantitative measurement of neutrophil CD64 expression in 41 patients undergoing total joint arthroplasty.
  • Blood samples collected pre-operatively and at 1, 3, 5, 7, and 14 days post-surgery.
  • Simultaneous measurement of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and white blood cell count (WBC).

More Related Videos

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice
09:34

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

Published on: June 22, 2016

Related Experiment Videos

Last Updated: May 8, 2026

Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model
12:23

Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model

Published on: April 24, 2020

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice
09:34

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

Published on: June 22, 2016

Main Results:

  • Neutrophil CD64 levels significantly elevated from day 1, peaked at day 3, and decreased significantly by day 5.
  • No post-operative infections were observed in the study cohort.
  • CD64 peak levels varied but were significantly influenced by baseline levels; some exceeded the infection cutoff value.

Conclusions:

  • Neutrophil CD64 levels exhibit a distinct pattern after uncomplicated total joint arthroplasty, rising sharply and then declining rapidly.
  • Understanding this normal post-operative CD64 kinetic is crucial for accurate early diagnosis of periprosthetic infections.
  • The established kinetics provide a reference for differentiating infection from normal inflammatory responses.