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Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Allergic Drug Reactions01:27

Allergic Drug Reactions

Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing numerous...
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

Overview
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Special Features of Adaptive Immunity01:20

Special Features of Adaptive Immunity

The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...

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Related Experiment Video

Updated: May 8, 2026

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
09:04

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Published on: April 19, 2017

Evaluation of T cell function in allergic disease.

Brianne R Barker1

  • 1Department of Biology, Drew university, Madison, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 15, 2013
PubMed
Summary

This study details methods for isolating and analyzing T lymphocytes, crucial for understanding allergic disease. Protocols cover T cell surface proteins, proliferation, and cytokine production to characterize T cell subsets.

Area of Science:

  • Immunology
  • Allergy Research
  • T cell Biology

Background:

  • T lymphocytes critically influence allergic disease pathogenesis.
  • Understanding T cell roles requires effective isolation and functional characterization.
  • Allergy models and therapies benefit from detailed T cell subset analysis.

Purpose of the Study:

  • To outline protocols for T lymphocyte isolation.
  • To describe methods for evaluating T cell activation and function.
  • To provide techniques for identifying T cell subsets in allergy research.

Main Methods:

  • T cell isolation from biological samples.
  • Flow cytometry for surface protein expression analysis.
  • Assays for T cell proliferation, cytokine production (intracellular staining, ELISPOT).

More Related Videos

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
10:39

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Published on: April 16, 2019

Related Experiment Videos

Last Updated: May 8, 2026

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
09:04

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Published on: April 19, 2017

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
10:39

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Published on: April 16, 2019

Main Results:

  • Established protocols for T cell isolation.
  • Methods for assessing T cell surface markers and proliferation.
  • Techniques for quantifying cytokine production to identify T cell subsets.

Conclusions:

  • Comprehensive protocols enable detailed T cell analysis in allergy.
  • Characterization of T cell subpopulations is vital for advancing allergy research.
  • These methods support the development of targeted allergy therapies.