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Related Experiment Video

Updated: May 8, 2026

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation
08:44

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Published on: May 30, 2020

Protocols for identifying, enumerating, and assessing mouse eosinophils.

Kimberly D Dyer1, Katia E Garcia-Crespo, Caroline M Percopo

  • 1Inflammation Immunobiology Section, Laboratory of Allergic Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 15, 2013
PubMed
Summary

This study details methods for isolating and assessing mouse eosinophils, crucial for understanding allergic diseases. Protocols are provided for reliable evaluation of eosinophil functions in mouse models.

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Last Updated: May 8, 2026

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Area of Science:

  • Immunology
  • Allergic Diseases Research

Background:

  • Eosinophils play a key role in allergic diseases.
  • Existing mouse models are widely used to study eosinophil functions.
  • Murine eosinophils differ structurally and functionally from human eosinophils.

Purpose of the Study:

  • To provide reliable methods for the detection, isolation, and functional assessment of mouse eosinophils.
  • To present a protocol for eosinophil growth and differentiation from mouse bone marrow progenitors.
  • To enable accurate evaluation of mouse eosinophil contributions in research.

Main Methods:

  • Development of specific protocols for mouse eosinophil isolation from tissue.
  • Establishment of methods for functional assessment of isolated mouse eosinophils.
  • A protocol for in vitro eosinophil differentiation from mouse bone marrow.

Main Results:

  • Validated methods for detecting and isolating mouse eosinophils.
  • Established protocols for functional assays applicable to mouse eosinophils.
  • Demonstrated successful growth and differentiation of eosinophils from mouse progenitors.

Conclusions:

  • The presented protocols offer a reliable framework for studying mouse eosinophils.
  • These methods address the limitations of direct translation from human eosinophil studies.
  • The research facilitates a better understanding of eosinophil roles in mouse models of disease.