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Related Experiment Video

Updated: May 8, 2026

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology
11:42

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

Peptide release, side-chain deprotection, work-up, and isolation.

Søren L Pedersen1, Knud J Jensen

  • 1IGM, Faculty of Life Sciences, University of Copenhagen, Gubra, Hørsholm, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|August 15, 2013
PubMed
Summary
This summary is machine-generated.

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This study details methods for cleaving synthesized peptides from solid supports, yielding various C-terminal functionalities like acids, amides, amines, and aldehydes. Protocols cover common linkers, purity determination, and storage for peptide synthesis.

Area of Science:

  • Peptide Chemistry
  • Solid-Phase Synthesis
  • Biochemical Techniques

Background:

  • Peptide synthesis requires releasing the final peptide from a solid support for further use or analysis.
  • The choice of linker and cleavage conditions dictates the C-terminal functionality of the liberated peptide.
  • Standard cleavage often removes side-chain protecting groups simultaneously, but sequential or orthogonal strategies exist.

Purpose of the Study:

  • To provide comprehensive protocols for releasing peptides from common solid-phase synthesis linkers.
  • To demonstrate the generation of diverse C-terminal peptide functionalities, including acids, amides, amines, and aldehydes.
  • To offer guidance on assessing peptide purity and appropriate storage conditions post-cleavage.

Main Methods:

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Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels

Published on: December 6, 2012

Related Experiment Videos

Last Updated: May 8, 2026

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology
11:42

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides
09:54

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides

Published on: August 20, 2018

Generation and Recovery of &beta;-cell Spheroids From Step-growth PEG-peptide Hydrogels
09:21

Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels

Published on: December 6, 2012

  • Utilized various established linker chemistries for solid-phase peptide synthesis.
  • Developed and optimized cleavage cocktails and conditions for orthogonal and graduated deprotection strategies.
  • Applied analytical techniques for purity assessment and established storage protocols.

    • Main Results:

    • Successfully released peptides with diverse C-terminal functionalities (acids, amides, amines, aldehydes) from commonly used linkers.
    • Demonstrated the efficacy of two-stage cleavage procedures using orthogonal chemistry or graduated lability.
    • Provided validated methods for peptide purity determination and recommended storage conditions.

    Conclusions:

    • Established versatile protocols for peptide cleavage from solid supports, enabling access to a wide range of C-terminal modifications.
    • The described methods facilitate the production of peptides with specific functionalities for diverse applications.
    • Recommendations for purity analysis and storage ensure the integrity and utility of synthesized peptides.