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Updated: May 8, 2026
Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain
Published on: April 28, 2022
In-depth proteomic analysis of mouse microglia using a combination of FASP and StageTip-based, high pH,
Dohyun Han1, Sungyoon Moon, Yikwon Kim
1Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul, Korea; Institute of Medical & Biological Engineering, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
Abstract:
Microglia are major immune cells in the central nervous system. A characterization of microglia proteome would facilitate on the study of microglial functions in association with various neurodegenerative diseases. To build a reference proteome, we established a BV-2 microglial proteome to a depth of 5494 unique protein groups using a novel strategy that combined FASP, StageTip-based high pH fractionation, and high-resolution MS quickly and cost efficiently. By bioinformatics analysis, the BV-2 proteome is a valuable resource for studies of microglial function, such as in the immune response, inflammatory response, and phagocytosis. All MS data have been deposited in the ProteomeXchange with identifier PXD000168.
Insights
Researchers characterized the BV-2 microglial proteome, identifying 5494 proteins. This proteomic dataset offers a valuable resource for studying microglial functions in neurodegenerative diseases.
Area of Science:
- Neuroscience
- Immunology
- Proteomics
Background:
- Microglia are key immune cells within the central nervous system.
- Understanding microglial proteomes is crucial for investigating their role in neurodegenerative diseases.
Purpose of the Study:
- To establish a comprehensive reference proteome for BV-2 microglial cells.
- To provide a resource for studying microglial functions, including immune and inflammatory responses.
Main Methods:
- A novel strategy combining FASP, StageTip-based high pH fractionation, and high-resolution mass spectrometry (MS) was employed.
- This approach enabled rapid and cost-efficient proteome characterization.
Main Results:
- A BV-2 microglial proteome comprising 5494 unique protein groups was successfully established.
- Bioinformatics analysis confirmed the proteome's utility for studying microglial functions.
Conclusions:
- The generated BV-2 microglial proteome serves as a valuable resource for neuroscientific and immunological research.
- This dataset aids in understanding microglial roles in immune response, inflammation, and phagocytosis, relevant to neurodegenerative conditions.
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