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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

DNA modification under mild conditions by Suzuki-Miyaura cross-coupling for the generation of functional probes

Lukas Lercher1, Joanna F McGouran, Benedikt M Kessler

  • 1Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA (UK).

Angewandte Chemie (International Ed. in English)
|August 15, 2013
PubMed

Keywords:
DNAcross-couplingoligonucleotidespalladiumphotoaffinity labeling

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...