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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: May 8, 2026

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Flow cytometry in hematological disorders.

Hara Prasad Pati¹, Sonal Jain

¹Department of Hematology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India. harappati@yahoo.co.in

Indian Journal of Pediatrics

|August 15, 2013

Summary

This summary is machine-generated.

Flow cytometry is a powerful diagnostic tool in medicine, especially for hematological disorders like leukemia and lymphomas. This review highlights its applications in both malignant and non-malignant pediatric blood conditions.

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Area of Science:

Clinical Medicine
Hematology
Immunology

Background:

Flow cytometry is a widely adopted technology in diagnostic medicine.
Its application is particularly significant in the diagnosis and management of hematological disorders.

Purpose of the Study:

To review the diverse applications of flow cytometry in hematology.
To emphasize its role in diagnosing and characterizing pediatric hematological disorders.

Main Methods:

Review of current literature on flow cytometry applications in hematology.
Focus on studies involving pediatric patients with blood disorders.

Main Results:

Flow cytometry is crucial for diagnosing, characterizing, and guiding therapy for acute leukemia and lymphomas.
Increasingly used for non-malignant disorders affecting red blood cells, platelets, and leukocytes.
Specific applications in pediatric hematological conditions are highlighted.

Conclusions:

Flow cytometry is an indispensable tool in modern hematology.
Its utility extends across malignant and non-malignant blood disorders, with a notable impact on pediatric care.