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Updated: May 8, 2026
A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
Published on: August 25, 2018
Human adipose-derived stem cells cultured in keratinocyte serum free medium: Donor's age does not affect the
Dah-Ching Ding1, Hsiang-Lan Chou, Wei-Ting Hung
1Department of Obstetrics and Gynecology, Buddhist Tzu Chi General Hospital; Tzu Chi University, Hualien, Taiwan. hidrchu@gmail.com.
Background:
Although donor age-related effects of characteristics of mesenchymal stem cells (MSC), such as a decrease in the proliferation and differentiation capacity and an increase of senescence and apoptosis, are evident, such effects are generally less prominent in adipose-derived stem cells (ASC). Using a hormone and growth factor rich medium (KFSM), this study cultured ASC from abdominal subcutaneous fat of 27 adult females in three age groups: 30-39 y, 40-49 y and 50-60 y, and investigated the growth and differentiation characteristics.
Results:
The derived ASC had an immunophenotype similar to that of bone marrow derived MSC (BMSC). They could be stably expanded with an average population doubling time of 21.5 ± 2.3 h. Other than a higher pre-adipogenic commitment and a lower adipogenic differentiation capability in ASC derived from the old age group, other characteristics including proliferation rate, doubling time, telomere length, as well as the osteogenic and chondrogenic differentiation capacity were the same regardless of the donor's age.
Conclusions:
The study demonstrates a promising proliferation and differentiation capabilities of ASC regardless of the donor's age. The compromised adipogenic potential in the older donors could be a benefit for their application in regeneration therapy.
