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Related Experiment Video

Updated: May 8, 2026

Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis
09:22

Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis

Published on: May 12, 2020

Polymicrobial versus monomicrobial keratitis: a retrospective comparative study.

Nigel C S Lim1, Dawn K A Lim, Manotosh Ray

  • 1Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.

Eye & Contact Lens
|August 16, 2013
PubMed
Summary
This summary is machine-generated.

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The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...

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Polymicrobial keratitis, often linked to systemic factors and larger infiltrates, shows reduced treatment success and antibiotic sensitivity compared to monomicrobial infections. Early suspicion is key for better outcomes.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Infectious Diseases

Background:

  • Keratitis, an inflammation of the cornea, can be caused by a single pathogen (monomicrobial) or multiple pathogens (polymicrobial).
  • Understanding the differences in risk factors, clinical presentation, and treatment response between these two forms is crucial for effective management.

Purpose of the Study:

  • To compare the risk factors, clinical characteristics, microbiological profile, and treatment outcomes of polymicrobial versus monomicrobial keratitis.
  • To identify indicators for suspecting polymicrobial keratitis.

Main Methods:

  • A retrospective comparative case series analyzed culture-positive keratitis cases from January 2002 to December 2010.
  • Polymicrobial keratitis involved two or more pathogens, while monomicrobial keratitis was solely Pseudomonas aeruginosa.

Related Experiment Videos

Last Updated: May 8, 2026

Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis
09:22

Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis

Published on: May 12, 2020

  • Demographics, risk factors, clinical and microbiological data, and treatment outcomes were assessed.

    • Main Results:

    • Twenty-one eyes each were included. Polymicrobial keratitis patients were older, with larger corneal infiltrates and longer resolution times.
    • Systemic and multiple risk factors were unique to polymicrobial cases. Medical treatment succeeded in 80.9% of polymicrobial cases versus 100% of monomicrobial.
    • Common pathogens included Pseudomonas aeruginosa and Candida albicans. Gram-negative bacteria showed sensitivity to gentamicin and ciprofloxacin; gram-positive bacteria were sensitive to ciprofloxacin and cefazolin.

    Conclusions:

    • Polymicrobial keratitis should be suspected in patients with multiple and systemic risk factors, and larger corneal infiltrates.
    • Contact lens wear was a common risk factor in both groups.
    • Polymicrobial keratitis is associated with a prolonged disease course and potentially reduced antibiotic sensitivity.