Related Experiment Video
Updated: May 8, 2026

08:57
Scalable Syntheses of Graphene Oxide and Reduced Graphene Oxide using Cascade Design Oxidation and Highly Basic Reduction Reactions
Published on: July 3, 2025
Contrasting modulation of enzyme activity exhibited by graphene oxide and reduced graphene
Xinjian Yang1, Chuanqi Zhao, Enguo Ju
1State Key laboratory of Rare Earth Resources Utilization and Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China. jren@ciac.ac.cn xqu@ciac.ac.cn.
Abstract:
Here we demonstrate that GO and RGO exhibit contrasting effects on modulation of the peroxidase activity of cyt c. GO could dramatically improve the enzyme activity of cyt c, while RGO showed a strong inhibition effect on cyt c.
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Enzyme Inhibition
Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
Allosteric Regulation
Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...

