[Study on all-time multi-wavelength fusion fingerprint of Qizhiweitong granules and multi-component quantitative
Dong Yao1, Xian-Sheng Meng, Shuai Wang
1College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. yaodong_2010@163.com
Objective:
To control the quality of Qizhiweitong granules with the all-time multi-wavelength fusion fingerprint quantification as the major technique.
Method:
Agilent TC-C18 (4.6 mm x 250 mm, 5 microm) chromatographic column was adopted, with 0.02% formic acid water-acetonitrile as the mobile phase for linear gradient elution. The flow rate was 1 mL x min(-1), column temperature was 30 degrees C, and detector wavelength was 230, 254, 283 nm. Matlab was adopted for all-time multiple-wavelength fusion for data in dif format.
Result:
A good relationship was shown for albiflorin in 56.5-452 mg x L(-1) (r = 0.999 8), paeoniflorin in 107-856 mg x L(-1) (r = 0.999 8), licorice glycoside in 73.4-687 mg x L(-1) (r = 0.999 8), naringin in 109-872 mg x L(-1) (r = 0.999 8), neohesperidin in 48.0-384 mg L(-1) (r = 0.999 8), and glycyrrhizic acid in 38.6-308 mg x L(-1) (r = 0.999 8), with recoveries of 0.999 8.
Conclusion:
The method is simple, accurate and highly reproducible, and can provide basis for quality control of Qizhiweitong granules.