Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The neuroendocrine polypeptide 7B2 is a precursor protein.

T A Ayoubi1, H L van Duijnhoven, W J van de Ven

  • 1Department of Animal Physiology, University of Nijmegen, The Netherlands.

The Journal of Biological Chemistry
|September 15, 1990
PubMed
Summary

Neuroendocrine protein 7B2 is synthesized as a 25 kDa precursor and processed into an 18 kDa form. Only the processed 18 kDa 7B2 is secreted from Xenopus laevis pituitary cells, suggesting an intracellular role for the precursor.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cloning and sequence analysis of hypothalamus cDNA encoding tilapia melanin-concentrating hormone.

Fish physiology and biochemistry·2013
Same author

Orexinergic innervation of urocortin1 and cocaine and amphetamine regulated transcript neurons in the midbrain centrally projecting Edinger-Westphal nucleus.

Journal of chemical neuroanatomy·2013
Same author

Cannabinoid modulation of midbrain urocortin 1 neurones during acute and chronic stress.

Journal of neuroendocrinology·2012
Same author

The amygdala, a relay station for switching on and off pain.

European journal of pain (London, England)·2012
Same author

Differential responses of corticotropin-releasing factor and urocortin 1 to acute pain stress in the rat brain.

Neuroscience·2011
Same author

Extracellular-signal regulated kinase regulates production of pro-opiomelanocortin in pituitary melanotroph cells.

Journal of neuroendocrinology·2010

Area of Science:

  • Neuroendocrinology
  • Molecular Biology
  • Protein Biosynthesis

Background:

  • The neuroendocrine protein 7B2 is conserved and found in neurons and endocrine cells.
  • 7B2 is coexpressed with proopiomelanocortin (POMC) in the Xenopus laevis pituitary intermediate lobe.

Purpose of the Study:

  • To investigate the biosynthesis and processing of the 7B2 protein in Xenopus laevis.
  • To determine the functional localization (intracellular vs. extracellular) of 7B2 and its derivatives.

Main Methods:

  • Immunoprecipitation analysis using an anti-7B2 monoclonal antibody.
  • Pulse and pulse-chase labeling of newly synthesized proteins.
  • Analysis of secreted proteins in incubation medium.
  • Tunicamycin treatment to assess glycosylation.

Related Experiment Videos

  • Chemical and enzymatic peptide mapping.

    • Main Results:

    • Newly synthesized 7B2 (25 kDa) was processed to an 18 kDa form during chase incubation.
    • Only the 18 kDa processed 7B2 was secreted and its secretion was dopamine-regulated.
    • Neither 7B2 nor its 18 kDa derivative were glycosylated, unlike POMC.
    • Peptide mapping indicated processing occurs in the carboxyl-terminal region of 7B2.

    Conclusions:

    • 7B2 functions as a precursor molecule with an intracellular role.
    • Extracellular functions are attributed to 7B2-derived peptides, not the intact 7B2 protein.
    • The processing and regulated secretion of 7B2 are key aspects of its biological activity.