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Updated: May 8, 2026
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Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
Published on: October 20, 2014
Transcriptional hierarchies regulating early blood cell development
Victoria Moignard1, Steven Woodhouse, Jasmin Fisher
1University of Cambridge, Department of Haematology, Wellcome Trust and MRC Cambridge Stem Cell Institute and Cambridge Institute for Medical Research, Cambridge, CB2 0XY, UK.
Blood Cells, Molecules & Diseases
|August 17, 2013
Summary
Hematopoiesis, the development of blood stem cells, is studied using mouse embryos and embryonic stem cells. Integrated genomic analysis reveals regulatory networks controlling blood formation from mesoderm.
Area of Science:
- Stem cell biology
- Developmental biology
- Genomics
Background:
- Hematopoiesis is a key model for stem cell research.
- Embryonic development of the hematopoietic system is not fully understood.
- Mouse embryos and embryonic stem cells offer complementary models for studying blood development.
Purpose of the Study:
- To review the embryonic origin of blood and regulatory networks.
- To analyze integrated genomic data from mouse embryos and ES cells.
- To reconstruct regulatory hierarchies in hematopoietic development.
Main Methods:
- Review of existing literature on embryonic hematopoiesis.
- Integrated analysis of genome-wide microarray data.
- Integrated analysis of ChIP-seq data from mouse embryos and ES cell lines.
Main Results:
- Identification of transcription factor networks regulating hematopoietic emergence from mesoderm.
- Reconstruction of regulatory hierarchies in hematopoietic development.
- Confirmation of known regulatory linkages and suggestion of novel interactions.
Conclusions:
- Embryonic stem cells provide a tractable model for studying hematopoietic development.
- Integrated genomic analysis is powerful for reconstructing regulatory networks.
- This approach advances our understanding of embryonic blood formation and stem cell regulation.
Related Concept Videos
01:20Overview of Hematopoiesis
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
01:21Hematopoiesis
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
01:21Lineage Commitment
Commitment is the process whereby stem cells:
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...