A simple method for protein electrophoresis of unconcentrated urine

Thirty-two unconcentrated urines were electrophoresed on cellogels in 0.05 M barbital buffer, pH 8.6, at 280 volts for 35 minutes. Instead of concentrating 100 fold, the urine samples were directly applied on the gel in volumes from 1.2 to 3.6 microliters and Coomassie dye was used for staining. This makes it possible to observe the protein patterns at trace concentration by reagent strips (about 0.05-0.2 g/l). This method is simple, convenient and suited for routine services.