Related Experiment Video

Updated: May 8, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

Draft Genome Sequence of Klebsiella pneumoniae Isolate PR04

M H Zulkifli1, L K Teh, L S Lee

  • 1Integrative Pharmacogenomics Institute (iPROMISE), Faculty of Pharmacy, Universiti Teknologi MARA (UiTM) Malaysia, Bandar PuncakAlam, Selangor DarulEhsan, Malaysia.

Genome Announcements
|August 17, 2013
PubMed

Insights

This study details the genome of Klebsiella pneumoniae PR04, a strain isolated from a Malaysian hospital patient. Its genomic structure differs from established Klebsiella pneumoniae reference strains.

Area of Science:

  • Microbiology
  • Genomics

Background:

  • Klebsiella pneumoniae is an opportunistic pathogen.
  • Antimicrobial resistance in Klebsiella pneumoniae is a growing public health concern.
  • Genomic characterization is crucial for understanding bacterial evolution and virulence.

Purpose of the Study:

  • To present the draft genome sequence of Klebsiella pneumoniae PR04.
  • To compare the genomic structure of K. pneumoniae PR04 with reference strains.

Main Methods:

  • Isolation of Klebsiella pneumoniae PR04 from a clinical sample.
  • Whole-genome sequencing.
  • Comparative genomic analysis.

Main Results:

  • The draft genome sequence of K. pneumoniae PR04 was successfully obtained.
  • Genomic structural differences were identified between K. pneumoniae PR04 and reference strains (MGH 78578, NTUH-K2044).

Conclusions:

  • The unique genomic structure of K. pneumoniae PR04 may influence its biological characteristics.
  • Further studies are warranted to explore the implications of these genomic variations.

More Related Videos

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient05:52

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

Published on: January 7, 2019

Related Concept Videos

Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...