Related Experiment Video
Updated: May 8, 2026
Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
Published on: March 12, 2021
Expression of multiple Transient Receptor Potential channel genes in murine 3T3-L1 cell lines and adipose tissue
Mahendra Bishnoi1, Kanthi Kiran Kondepudi, Aakriti Gupta
1National Agri-Food Biotechnology Institute (NABI), SAS Nagar (Mohali)-160071, Punjab, India. mbishnoi@gmail.com
Background:
Calcium and its signaling have a role in adipogenesis. Transient Receptor Potential (TRP) channels are non-selective cation channels with a high permeability to calcium.
Methods:
In the present study the expression of multiple TRP channels on mouse 3T3-L1 preadipocyte and adipocyte cells, white (WAT) and brown (BAT) adipose tissues was investigated using real time PCR (RT-PCR).
Results:
TRPV1, TRPV3, TRPM8, TRPC4, TRPC6 were differentially expressed in preadipocytes and adipocytes suggesting their significance in adipogenesis. Genes for multiple TRP channels were also expressed in murine WAT and BAT, out of which TRPV4, TRPV6 and TRPC6 showed differential expression.
Conclusion:
Present study demonstrates the expression of TRP channels in mouse cell lines and adipose tissues.
