Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

Forced Transdifferentiation

Forced Transdifferentiation

Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial transdifferentiation occurs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dysautonomia and joint hypermobility reflect a distinct subtype in eosinophilic esophagitis.

The Journal of allergy and clinical immunology·2026

Same author

Latency-Associated Peptide Rapidly Upregulates Neuraminidase 3 in a Profibrotic Translation-Based Positive Feedback Loop.

Cells·2026

Same author

Phosphoproteomic Profiling Reveals Overlapping and Distinct Signaling Pathways in <i>Dictyostelium discoideum</i> in Response to Two Different Chemorepellents.

Cells·2026

Same author

Pharmacological inhibition of host pathways enhances macrophage killing of intracellular bacterial pathogens.

Microbiology spectrum·2025

Same author

Phosphoproteomic Profiling Reveals Overlapping and Distinct Signaling Pathways in <i>Dictyostelium discoideum</i> in Response to two different chemorepellents.

bioRxiv : the preprint server for biology·2025

Same author

Differences between human male and female neutrophils with respect to which RNAs are present in polysomes.

bioRxiv : the preprint server for biology·2025

Search research articles

Related Experiment Video

Updated: May 8, 2026

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

Trypsin potentiates human fibrocyte differentiation.

Michael J V White¹, Melissa Glenn, Richard H Gomer

¹Department of Biology, Texas A&M University, College Station, Texas, United States of America.

|August 17, 2013

Summary

This summary is machine-generated.

Topical trypsin accelerates wound healing by promoting monocyte to fibrocyte differentiation. This process involves albumin fragments generated by trypsin, enhancing wound repair mechanisms.

More Related Videos

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Published on: August 20, 2021

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

Published on: April 3, 2021

Related Experiment Videos

Last Updated: May 8, 2026

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Published on: August 20, 2021

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

Published on: April 3, 2021

Area of Science:

Biochemistry
Cell Biology
Wound Healing Research

Background:

Monocytes differentiate into fibrocytes, crucial for granulation tissue formation and wound healing.
The precise mechanism by which trypsin in topical treatments enhances wound healing remains unclear.

Purpose of the Study:

To elucidate the mechanism of action for trypsin in promoting wound healing.
To investigate trypsin's effect on monocyte differentiation into fibrocytes.

Main Methods:

Human monocytes were cultured with varying concentrations of trypsin (20-200 ng/ml).
The role of trypsin's proteolytic activity was assessed using trypsin inhibitors.
The necessity of albumin and the effect of albumin fragments were examined.

Main Results:

Trypsin significantly potentiated the differentiation of human monocytes into fibrocytes at concentrations found in wound dressings.
This potentiation was dependent on trypsin's proteolytic activity.
Albumin was required for this effect, and tryptic fragments of albumin also promoted fibrocyte differentiation.

Conclusions:

Topical trypsin likely accelerates wound healing by generating albumin fragments.
These albumin fragments potentiate monocyte-to-fibrocyte differentiation, a key step in wound repair.