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Related Experiment Video

Updated: May 8, 2026

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

Published on: May 17, 2020

A transgenic tri-modality reporter mouse.

Xinrui Yan¹, Pritha Ray, Ramasamy Paulmurugan

¹Departments of Radiology, MIPS and Bio-X, Stanford University, Stanford, California, USA.

|August 17, 2013

Summary

This summary is machine-generated.

We developed a Tri-Modality Reporter Mouse for tracking stem cells, tissues, and organs using bioluminescence, fluorescence, and PET imaging. This versatile tool enables precise monitoring in various research applications, including transplantation and tissue engineering.

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Area of Science:

Biotechnology
Molecular Imaging
Genetics

Background:

Transgenic mice are valuable for uniformly labeled cells and tissues.
Existing reporter systems may lack multi-modality capabilities for comprehensive tracking.

Purpose of the Study:

To develop a novel transgenic mouse model for multi-modal imaging of cells and tissues.
To validate the utility of this reporter system in tracking mesenchymal stem cells (MSCs) in vivo.

Main Methods:

Generated a transgenic mouse expressing a tri-fusion reporter gene (fluc2-tdTomato-ttk) under a constitutive promoter.
Validated reporter gene expression linearity across bioluminescent, fluorescent, and PET modalities in vitro and in vivo.
Assessed MSC tracking and therapeutic efficacy in myocardial infarction and ischemic limb models.

Main Results:

Demonstrated linear correlation between all three reporter modalities (R² > 0.89).
Showed linear correlation between reporter signals and cell numbers for MSCs (R² > 0.99).
Confirmed successful in vivo tracking of MSCs in myocardial infarction and ischemic limb models, with improved perfusion in the latter.

Conclusions:

The Tri-Modality Reporter Mouse provides a robust platform for multi-modal cell and tissue tracking.
This system is highly applicable for stem cell research, tissue engineering, and organ transplantation studies.
The reporter system enables quantitative assessment of cell engraftment and therapeutic outcomes.