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Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

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Updated: May 8, 2026

Pyrosequencing for Microbial Identification and Characterization
12:37

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

Microbial analysis in primary and persistent endodontic infections by using pyrosequencing.

Bo-Young Hong1, Tae-Kwon Lee, Sang-Min Lim

  • 1Division of Periodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, Connecticut, USA.

Journal of Endodontics
|August 20, 2013
PubMed
Summary

Persistent endodontic infections show similar bacterial diversity to primary infections. Pyrosequencing analysis revealed no significant differences in the intracanal microbiota community structure between untreated and root-filled samples.

Keywords:
Diversityoperational taxonomic unitpersistent infectionprimary infectionpyrosequencingrichness

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Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
09:55

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Published on: May 2, 2018

Area of Science:

  • Microbiology
  • Endodontics
  • Genomics

Background:

  • Asymptomatic chronic apical periodontitis can result from primary or persistent endodontic infections.
  • Understanding the intracanal microbiota is crucial for effective endodontic treatment.

Purpose of the Study:

  • To investigate and compare the bacterial community profiles of intracanal microbiota in primary versus persistent endodontic infections.
  • To test the hypothesis that there is no difference in bacterial community diversity between these infection types.

Main Methods:

  • GS-FLX Titanium pyrosequencing was used to analyze 16S rRNA gene sequences.
  • Samples included 10 untreated (primary) and 8 root-filled (persistent) endodontic infections.
  • Bioinformatic analysis identified operational taxonomic units, genera, and phyla.

Main Results:

  • Analysis of 18 samples yielded over 124,000 16S rRNA gene sequences.
  • Bacteroidetes was the most abundant phylum in both infection groups.
  • No significant differences in bacterial diversity, structure, or composition were found between primary and persistent infections (P > .05).

Conclusions:

  • Persistent endodontic infections harbor a bacterial community as diverse as primary infections.
  • The findings suggest similar microbial profiles regardless of infection persistence status.