Specificity data of the tricyclic antidepressants assay by fluorescent polarization immunoassay

Additional specificity data of the fluorescent polarization immunoassay (FPIA) for tricyclic antidepressants (TCA) in serum were evaluated. The TDx TCA assay exhibits cross-reactivities for a variety of the tricyclic antidepressants, tetracyclic antidepressants, phenothiazines, and several metabolites from these compounds. Significant cross-reactivities were found for dixyrazine (20-40%), alimemazine (20-47%), perazine (23-33%), Iofepramine (27-30%), desmethyltrimipramine (40-57%), opipramol (60-77%), amitriptylinoxide (73-85%), and dothiepin (97-113%).