Related Experiment Video
Updated: May 8, 2026
12:33
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
Analysis of three leafminers' complete mitochondrial genomes
Fei Yang1, Yuzhou Du, Jingman Cao
1College of Horticulture and Plant Protection and Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, PR China; Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
Gene
|August 20, 2013
Summary
This study sequenced the mitochondrial genomes of three major leafminer pests: Liriomyza trifolii, Liriomyza huidobrensis, and Liriomyza bryoniae. The genomic data offers valuable insights for pest identification and understanding Diptera phylogeny.
Area of Science:
- Genomics
- Entomology
- Molecular Biology
Background:
- Liriomyza trifolii, Liriomyza huidobrensis, and Liriomyza bryoniae are significant global leafminer pests.
- Understanding their genetic makeup is crucial for effective pest management.
Purpose of the Study:
- To sequence and analyze the complete mitochondrial genomes of L. trifolii, L. huidobrensis, and L. bryoniae.
- To identify genetic markers for species identification and phylogenetic analysis.
Main Methods:
- Whole-genome sequencing of three Liriomyza species.
- Bioinformatic analysis of mitochondrial genome structure and gene content.
- Comparative analysis of A+T-rich regions and ribosomal RNA genes.
Main Results:
- Complete mitochondrial genomes of L. trifolii (16,141 bp), L. huidobrensis (16,236 bp), and L. bryoniae (16,183 bp) were determined.
- All genomes contained 37 typical animal mitochondrial genes and an A+T-rich region, with high compactness.
- Significant variations were observed in the A+T-rich regions, while ribosomal RNA genes showed limited variability.
Conclusions:
- The mitochondrial genome data provides a foundation for studying Diptera phylogeny.
- Genetic markers derived from these genomes can aid in the identification of leafminer species.
- Ribosomal RNA genes are not ideal for population genetics studies of these leafminers.
Keywords:
ATP synthase F0 subunit 6ATP synthase F0 subunit 8DipteraGenomeIleIntroduced SpeciesIsoleucineLeuLeucineMitochondrialNADH dehydrogenase subunit 1NADH dehydrogenase subunit 2NADH dehydrogenase subunit 3NADH dehydrogenase subunit 4NADH dehydrogenase subunit 4lNADH dehydrogenase subunit 5NADH dehydrogenase subunit 6PhePhenylalanineSerSerineatp6atp8cobcox1cox2cox3cytochrome bcytochrome c oxidase subunit 1cytochrome c oxidase subunit 2cytochrome c oxidase subunit 3mitochondrial 12s ribosomal rnamitochondrial 16s ribosomal rnanad1nad2nad3nad4nad4lnad5nad6rrnLrrnS
Related Concept Videos
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
02:54Evolutionary Relationships through Genome Comparisons
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
01:29Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
02:19Export of Mitochondrial and Chloroplast Genes
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred irrespective...
02:59Animal Mitochondrial Genetics
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
01:28Microbial Phylogeny
Understanding the evolutionary relationships among microorganisms is fundamental to microbial ecology and taxonomy. Phylogenetic trees are essential tools for inferring these relationships, relying primarily on comparative analyses of molecular sequences such as DNA, RNA, or proteins. In microbial studies, these trees typically depict the evolutionary paths of diverse bacterial and archaeal species by mapping genetic differences accumulated over time.Phylogenetic trees are composed of tips,...