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Strategies to construct null and conditional null Trypanosoma brucei mutants using Cre-recombinase and loxP

Hee-Sook Kim1, Zhen Li, Catharine Boothroyd

  • 1Laboratory of Molecular Parasitology, The Rockefeller University, New York, NY 10065, USA; Laboratory of Lymphocyte Biology, The Rockefeller University, New York, NY 10065, USA.

Molecular and Biochemical Parasitology
|August 20, 2013
PubMed

Keywords:
ALDBLEBSDConditional gene knockoutCre-recombinaseGCVGene knockoutHSVTK or TKHYGHerpes simplex virus thymidine kinaseKONEOORFPURSASTUBTrypanosoma bruceiUTRaldolaseblasticidin-resistance genebleomycin-resistance genegancyclovirhygromycin-resistance geneknockoutneomycin-resistance geneopen reading framepuromycin-resistance genesplice accepter sitetubulinuntranslated region

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