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Updated: May 8, 2026
Micro-Colony Forming Unit Assay for Efficacy Evaluation of Vaccines Against Tuberculosis
Published on: July 28, 2023
Cytokine responses to quantiferon peptides in pediatric tuberculosis: a pilot study
Marine Armand1, Vibol Chhor, Agathe de Lauzanne
1Laboratory of Immunology, Robert Debré Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Univ. Paris Diderot, Sorbonne Paris Cité, 75019 Paris, France.
Objectives:
Detailed understanding of tuberculosis (TB) immunopathology and cytokine/chemokine responses can ultimately lead to the development of new diagnostic tools, especially useful in children where TB diagnosis remains challenging.
Methods:
Nineteen cytokine/chemokine responses to Mycobacterium tuberculosis (M.tb) antigens were analyzed in 47 children distributed as follow: 28 with TB-disease (TD), 12 with latent TB and 7 uninfected controls. All the cytokines and chemokines were quantified in a multiplexed microsphere-based assay by using residual plasma from the quantiFERON kit (IFNγ release assay).
Results:
IP-10, IL-2, IL-5 and IL-13 were among the best cytokines to diagnose infection as related by the area under ROC curve for IP-10 (0.96, 95%CI: 0.91-1.00), IL-2 (0.98, 95%CI: 0.93-1.02), IL-5 (0.91, 95%CI: 0.81-1.01) and IL-13 (0.97, 95%CI: 0.93-1.00). None of the 5 biomarkers, however, discriminated TB-disease from latent-TB. Finally, lower IL-5 (p = 0.02) and IL-13 (p = 0.02) levels were observed in severe opposed to non-severe TB.
Conclusion:
These results suggest that IP-10, IL-2, IL-5 and IL-13 may find a diagnostic application in pediatric tuberculosis and argue against the paradigm of a negative influence of Th2 responses in severe pediatric M.tb infection.
