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Related Experiment Video

Updated: May 8, 2026

Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
08:37

Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue

Published on: May 28, 2015

DNA methylation markers in the postnatal developing rat brain.

Rebecca K Simmons1, Sara A Stringfellow, Matthew E Glover

  • 1Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham, Birmingham, AL, USA.

Brain Research
|August 20, 2013
PubMed
Summary
This summary is machine-generated.

DNA methylation processes are crucial for brain development. This study reveals dynamic changes in DNA methyltransferases (DNMTs) and global methylation during early postnatal rat brain development, varying by region.

Keywords:
AmygdalaDNA methylationDNA methyltransferaseHippocampusIn situ hybridizationNeurodevelopment

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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
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Last Updated: May 8, 2026

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Published on: May 28, 2015

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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Area of Science:

  • Neuroscience
  • Epigenetics
  • Developmental Biology

Background:

  • Altered epigenetic processes, including DNA methylation, are implicated in psychiatric and neurodevelopmental disorders.
  • Understanding DNA methylation changes during early brain development is limited.

Purpose of the Study:

  • To investigate the developmental changes in DNA methyltransferases (DNMTs) and global DNA methylation in the rat brain during early postnatal development.
  • To examine regional differences in these epigenetic changes.

Main Methods:

  • In situ hybridization was used to assess mRNA expression of DNMT1, DNMT3a, and DNMT3b at seven postnatal timepoints.
  • Global DNA methylation levels (5-methylcytosine) were measured in selected brain regions during the first three postnatal weeks.

Main Results:

  • DNMT mRNA expression was widespread in the developing rat brain, peaking in the first week and declining over the first three postnatal weeks in several regions.
  • Global DNA methylation levels showed a gradual increase in the hippocampus and remained stable in the amygdala and prefrontal cortex, not mirroring DNMT mRNA trends.
  • Significant regional variations in DNA methylation patterns were observed during early development.

Conclusions:

  • DNA methylation dynamics exhibit distinct developmental trajectories across different brain regions.
  • These findings provide insights into the epigenetic landscape of the developing brain and its potential relevance to neurodevelopmental disorders.