Jove
Visualize
Contact Us

Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Removal of dsRNA byproducts using affinity chromatography.

Molecular therapy. Nucleic acids·2025
Same author

An immuno-northern technique to measure the size of dsRNA byproducts in in vitro transcribed RNA.

Electrophoresis·2024
Same author

Functional Deimmunization of Botulinum Neurotoxin Protease Domain via Computationally Driven Library Design and Ultrahigh-Throughput Screening.

ACS synthetic biology·2023
Same author

Building blocks and blueprints for bacterial autolysins.

PLoS computational biology·2021
Same author

Bioinformatics-driven discovery of novel Clostridioides difficile lysins and experimental comparison with highly active benchmarks.

Biotechnology and bioengineering·2021
Same author

Nonclassical antagonism between human lysozyme and AMPs against Pseudomonas aeruginosa.

FEBS open bio·2021
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 8, 2026

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
11:31

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Published on: June 30, 2016

A high-throughput screen for antibiotic drug discovery.

Thomas C Scanlon1, Sarah M Dostal, Karl E Griswold

  • 1Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, 03755.

Biotechnology and Bioengineering
|August 20, 2013
PubMed
Summary

This study introduces an ultra-high-throughput screening platform for discovering novel natural product antibiotics. The technology enables rapid screening of millions of clones daily, accelerating the search for new antibacterial drugs.

Keywords:
antibioticsdrug discoveryhigh-throughput screeningin vitro compartmentalizationmetagenomicsmicrofluidics

More Related Videos

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence
07:25

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence

Published on: February 23, 2021

Assay Development for High-Throughput Drug Screening Against Mycobacteria
07:50

Assay Development for High-Throughput Drug Screening Against Mycobacteria

Published on: October 25, 2024

Related Experiment Videos

Last Updated: May 8, 2026

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
11:31

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Published on: June 30, 2016

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence
07:25

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence

Published on: February 23, 2021

Assay Development for High-Throughput Drug Screening Against Mycobacteria
07:50

Assay Development for High-Throughput Drug Screening Against Mycobacteria

Published on: October 25, 2024

Area of Science:

  • Microbiology
  • Biotechnology
  • Drug Discovery

Background:

  • The urgent need for novel antibiotics to combat rising antimicrobial resistance.
  • Limitations of current screening methods in terms of throughput and efficiency.
  • The potential of natural products as a source for new antibacterial agents.

Purpose of the Study:

  • To develop and validate an ultra-high-throughput screening platform for natural product antibiotic discovery and engineering.
  • To enable rapid screening of millions of microbial clones for antibiotic activity.
  • To facilitate the identification of novel antibacterial compounds against human pathogens.

Main Methods:

  • Creation of hydrogel-in-oil emulsions encapsulating recombinant microorganisms and bacterial pathogens.
  • Assay of antibiotic activity using a fluorescent viability dye within hydrogel microdroplets.
  • Utilized bulk emulsification and microfluidic technology for droplet generation, compatible with flow cytometry.
  • Achieved droplet synthesis and sorting rates exceeding 3,000 drops/second.

Main Results:

  • Screening throughputs exceeding 5 million clones per day were achieved.
  • Demonstrated efficient selection of antibiotic-secreting yeast from a large background population.
  • Successfully screened a metagenomic library for secreted antibiotics targeting Staphylococcus aureus.
  • Hydrogel droplets of approximately 25 pL were synthesized and sorted.

Conclusions:

  • The developed platform offers a practical and accessible method for discovering and engineering natural product antibiotics.
  • The high-throughput nature of the system significantly accelerates the identification of novel antibacterial biomolecules.
  • This technology is expected to empower researchers in the pursuit of next-generation antibiotics.