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Updated: May 8, 2026

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
Published on: October 14, 2016
Hypersensitivity reaction to high-dose methotrexate and successful rechallenge in a pediatric patient with
Jeffrey R Scott1, Deborah A Ward, Kristine R Crews
1Pharmaceutical Department, St. Jude Children's Research Hospital, Memphis, Tennessee.
Abstract:
Hypersensitivity reactions to methotrexate are rare, but have been reported. Methotrexate has shown activity against many malignancies, and omission of methotrexate therapy may increase the risk of cancer-related death in some patients. Therefore, rechallenging patients with methotrexate following hypersensitivity may be beneficial. We report a case of a child with metastatic osteosarcoma who experienced a hypersensitivity reaction to high-dose methotrexate and was successfully rechallenged with methotrexate using a 6-hour infusion. Using this regimen, adequate peak methotrexate plasma concentrations were achieved and no further hypersensitivity reactions were noted.
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