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Related Experiment Videos

A modified plaque method for arboviruses on plastic panels.

V Hronovský, R Benda, V Plaisner

    Acta Virologica
    |April 1, 1975
    PubMed
    Summary
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    New culture media with higher succinate buffer concentrations enable longer cell cultivation. This led to an economical plaque titration method for arboviruses, proving sensitive and effective for plaque reduction tests.

    Area of Science:

    • Cell biology
    • Virology
    • Biotechnology

    Background:

    • Long-term cell cultivation is crucial for virological studies.
    • Standard cell culture methods can be limiting for arbovirus titration.
    • Developing cost-effective and sensitive titration techniques is essential.

    Purpose of the Study:

    • To develop an economical and sensitive method for arbovirus plaque titration.
    • To evaluate the efficacy of autoclavable culture media with enhanced succinate buffer for cell cultivation.
    • To compare the developed plaque titration method with traditional techniques.

    Main Methods:

    • Utilizing autoclavable culture media with a 10-15 fold increased concentration of succinate buffer.
    • Cultivating cells with free gas exchange in the developed media.

    Related Experiment Videos

  • Developing a plaque titration technique on plastic panels with methylcellulose overlay.
  • Testing the method with seven arboviruses from three groups and three cell lines (CV-1, PS, PK).

    • Main Results:

    • The enhanced culture media permitted comparatively long-term cell cultivation.
    • The developed plaque titration method demonstrated high sensitivity.
    • The method showed comparable or superior sensitivity to titrations in mice or tube cell cultures.
    • The technique was suitable for plaque reduction tests.

    Conclusions:

    • Autoclavable culture media with increased succinate buffer concentration facilitate extended cell cultivation.
    • An economical and sensitive plaque titration method for arboviruses was successfully developed.
    • This novel method is effective for arbovirus characterization and plaque reduction assays.