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Highly Efficient Electroporation-mediated Transformation into Edible Mushroom Flammulina velutipes

Jong Kun Kim1, Young Jin Park, Won Sik Kong

  • 1Graduate School of Biotechnology & Information Technology, Hankyong National University, Ansung 456-749, Korea. ; Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Korea.

Mycobiology
|August 20, 2013
PubMed

Keywords:
ElectroporationFlammulina velutipesFlammutoxin geneHygromycin B geneProtoplast

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In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
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