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Interaction of plasma membranes with influenza virus. V. Changes in creatine phosphokinase activity
Abstract:
A decrease in creatine phosphokinase (CPK) activity was observed in plasma membranes (PM) treated with native A/WSN influenza virus but not in PM treated with heat-inactivated virus. The decrease in CPK activity depended on the amount of virus added to PM, on the pH of the medium and on the quality of the isolated PM. It was evident already after 10 minutes of incubation at 37 degrees C. The possible mechanism of the inhibition of the CPK activity and the relation to changes in adenosine triphosphatase (ATPase) activity and to fusion are discussed.
Insights
Native influenza virus inhibits creatine phosphokinase (CPK) activity in plasma membranes (PM). This effect depends on virus concentration, pH, and PM quality, occurring rapidly at 37°C.
Area of Science:
- Virology
- Biochemistry
- Cell Biology
Background:
- Influenza viruses are significant human pathogens.
- Plasma membranes (PM) are crucial cellular barriers.
- Creatine phosphokinase (CPK) is an enzyme involved in cellular energy homeostasis.
Purpose of the Study:
- To investigate the effect of native A/WSN influenza virus on CPK activity in PM.
- To determine the factors influencing this interaction.
- To explore potential mechanisms and related enzymatic changes.
Main Methods:
- Incubation of isolated PM with native and heat-inactivated A/WSN influenza virus.
- Measurement of CPK activity under varying conditions (virus concentration, pH, temperature).
- Assessment of adenosine triphosphatase (ATPase) activity and membrane fusion.
Main Results:
- Native influenza virus significantly decreased CPK activity in PM.
- Heat-inactivated virus did not affect CPK activity.
- The inhibition was dependent on virus amount, pH, and PM quality, observed within 10 minutes at 37°C.
Conclusions:
- Native influenza virus directly impacts CPK activity in PM.
- The interaction suggests a specific viral mechanism affecting membrane-associated enzymes.
- Further research is needed to elucidate the precise mechanism and its link to viral fusion and ATPase activity.