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Related Experiment Video

Updated: May 8, 2026

Histological-Based Stainings Using Free-Floating Tissue Sections

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

Histochemistry: historical development and current use in pathology.

M A Riva¹, M Manzoni, G Isimbaldi

¹Section of History of Medicine, Research Centre on Public Health.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission

|August 21, 2013

Summary

Histochemical stains have a rich history in pathology. Modern labs use them less frequently than immunohistochemical (IHC) techniques, with a notable difference in naming conventions.

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Area of Science:

Histochemistry
Pathology
Medical History

Background:

Histochemical stains have been pivotal in advancing modern pathology over the past two centuries.
This study traces the chronological and geographical development of key histochemical stains.
The research examines the evolution and application of these stains in diagnostic practice.

Observation:

Classical histochemical stains are less prevalent in contemporary pathology laboratories compared to newer methods.
A significant shift towards immunohistochemical (IHC) techniques is observed.
The ratio of histochemical reactions to IHC reactions is approximately 1:13.

Findings:

The historical development of histochemistry is detailed, including the year, country, and primary application of major stains.
A quantitative analysis reveals the declining usage of traditional histochemical stains in modern diagnostics.
A distinct difference in terminology is identified between histochemical and IHC stains.

Implications:

Understanding the historical trajectory of histochemical stains provides context for current diagnostic practices.
The findings highlight the increasing dominance of IHC techniques in pathology.
The study suggests a cultural divergence in stain nomenclature, with histochemical stains often named eponymously and IHC stains using more objective biological terms.