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Updated: May 8, 2026
Mouse Models Of Helicobacter Infection And Gastric Pathologies
Published on: October 18, 2018
Comparing the genomes of Helicobacter pylori clinical strain UM032 and Mice-adapted derivatives
Yalda Khosravi1, Vellaya Rehvathy1, Wei Yee Wee2
1Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia.
Background:
Helicobacter pylori is a Gram-negative bacterium that persistently infects the human stomach inducing chronic inflammation. The exact mechanisms of pathogenesis are still not completely understood. Although not a natural host for H. pylori, mouse infection models play an important role in establishing the immunology and pathogenicity of H. pylori. In this study, for the first time, the genome sequences of clinical H. pylori strain UM032 and mice-adapted derivatives, 298 and 299, were sequenced using the PacBio Single Molecule, Real-Time (SMRT) technology.
Result:
Here, we described the single contig which was achieved for UM032 (1,599,441 bp), 298 (1,604,216 bp) and 299 (1,601,149 bp). Preliminary analysis suggested that methylation of H. pylori genome through its restriction modification system may be determinative of its host specificity and adaptation.
Conclusion:
Availability of these genomic sequences will aid in enhancing our current level of understanding the host specificity of H. pylori.
Insights
Sequencing the genomes of Helicobacter pylori strains, including mouse-adapted variants, reveals insights into bacterial adaptation. This research provides a foundation for understanding H. pylori host specificity and pathogenesis.
Area of Science:
- Microbiology
- Genomics
- Bacterial Pathogenesis
Background:
- Helicobacter pylori is a Gram-negative bacterium causing chronic stomach inflammation.
- Understanding H. pylori pathogenesis mechanisms is crucial.
- Mouse models are vital for studying H. pylori immunology and pathogenicity.
Purpose of the Study:
- To sequence the genomes of clinical H. pylori strain UM032 and its mice-adapted derivatives (298 and 299).
- To investigate potential mechanisms of H. pylori host specificity and adaptation.
Main Methods:
- PacBio Single Molecule, Real-Time (SMRT) sequencing technology was employed.
- Whole genome sequencing was performed on three H. pylori strains.
Main Results:
- Complete single contig genome sequences were obtained for UM032 (1,599,441 bp), 298 (1,604,216 bp), and 299 (1,601,149 bp).
- Preliminary analysis indicates that genome methylation via the restriction-modification system may influence H. pylori host specificity and adaptation.
Conclusions:
- The generated genomic sequences enhance understanding of H. pylori host specificity.
- This data will facilitate further research into H. pylori adaptation mechanisms.
