Related Experiment Video

Updated: May 8, 2026

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
10:08

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Published on: December 9, 2022

Organic cation transporter 2 (SLC22A2), a low-affinity and high-capacity choline transporter, is preferentially

T Nakata1, T Matsui, K Kobayashi

  • 1Department of Health Science, Ishikawa Prefectural Nursing University, 1-1 Gakuendai, Kahoku, Ishikawa 929-1210, Japan; Department of Anatomy and Neurobiology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.

Neuroscience
|August 21, 2013
PubMed

Keywords:
3,3′-diaminobenzidineAchAchRBTXCACCCNSChATCoDABDMEMDPXDulbecco’s modified Eagle’s mediumERFCSHC-3HRPIsKLHMBSNGSNMJsOCT2OCTsOsPBPBSPCSLC22A2SVsTMVAchTacetyl choline receptorsacetylcholinealpha-bungarotoxincentral autonomic cellscentral canalcentral nervous systemcholine acetyltransferasecholinergic neuroncortexdibutyl phthalate xyleneendoplasmic reticulumfetal calf serumhemicholiniumhorseradish peroxidaseinner stripe of the outer medullakeyhole limpet hemocyaninm-maleimidobenzoyl-N-hydroxysuccinimide esterneuromuscular junctionsnormal goat serumorganic cation transporterorganic cation transportersouter stripe of the outer medullapartition cellsphosphate bufferphosphate-buffered salinesynaptic vesiclesynaptic vesiclestransmembranevesicular acetyl choline transporter

More Related Videos

High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies07:10

High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies

Published on: September 29, 2023

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters08:55

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Published on: March 7, 2019

Related Concept Videos

Secondary Active Transport01:32

Secondary Active Transport

One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
Secondary Active Transport01:55

Secondary Active Transport

One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
Secondary Active Transport01:32

Secondary Active Transport

One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
Membrane Transporters01:31

Membrane Transporters

Transporters are essential membrane transport proteins with functions related to cell nutrition, homeostasis, communication, etc. Approximately 7% of all genes in the human genome code for transporters or transporter-related proteins.
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
Cholinergic Neurons: Neurotransmission01:23

Cholinergic Neurons: Neurotransmission

Cholinergic neurotransmission involves the synthesis and the release of acetylcholine (ACh) in order to transmit nerve impulses across the synapse. The process begins with the synthesis of acetyl CoA, a precursor for ACh, from ATP, acetate, and coenzyme A in the mitochondria. Choline, another vital precursor, is transported inside the neuron through choline transporters, including high-affinity choline transporter CHT1, low-affinity choline transporter CTL1, and lower-affinity choline...
Active Transport01:14

Active Transport

Active transport is a critical biological process that allows cells to move solutes against an electrochemical gradient. This process requires direct energy input and is characterized by its selectivity, saturability, and susceptibility to competitive inhibition.
Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...