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Mesenchymal stem cell derived microvesicles: trophic shuttles for enhancement of sperm quality parameters

Aram Mokarizadeh1, Mohammad-Amin Rezvanfar, Kamran Dorostkar

  • 1Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.

Reproductive Toxicology (Elmsford, N.Y.)
|August 21, 2013
PubMed

Insights

Mesenchymal stem cell-derived microvesicles (MVs) can improve cryopreserved sperm quality. Treatment with MSC-MVs enhanced sperm viability, motility, and adhesive properties, aiding fertility preservation.

Area of Science:

  • Reproductive Biology
  • Stem Cell Therapy
  • Cryopreservation Science

Background:

  • Sperm cryopreservation often diminishes fertilizing potential.
  • Mesenchymal stem cell-derived microvesicles (MSCs MVs) show regenerative capabilities.
  • MSC MVs shuttle trophic molecules aiding cellular repair.

Purpose of the Study:

  • To investigate the potential of MSC-derived MVs in rehabilitating cryopreserved rat sperm.
  • To assess the impact of MSC MVs on sperm viability, motility, and antioxidant capacity.
  • To evaluate changes in sperm surface adhesion molecules post-MSC MV treatment.

Main Methods:

  • Rat sperm were treated with varying concentrations of MSC-derived MVs (25, 50, 100μg) before cryopreservation.
  • Cryopreserved sperm were analyzed for viability, motility, and antioxidant parameters.
  • Flow cytometry was used to assess expression of surface adhesion molecules (CD29, CD44, ICAM-I, VCAM-I).

Main Results:

  • MSC MV treatment significantly enhanced sperm viability and motility.
  • Antioxidant capacity of cryopreserved sperm improved following MSC MV application.
  • Expression of key surface adhesion molecules involved in sperm function was upregulated.

Conclusions:

  • MSC-derived MVs show promise in improving the quality of cryopreserved sperm.
  • This approach could mitigate fertility loss associated with sperm cryopreservation.
  • MSC MVs represent a potential therapeutic strategy for enhancing sperm fertilizing potential.
Keywords:
Antioxidant capacityCryopreservationMesenchymal stem cell-derived microvesiclesMotilityReproductive toxicitySpermViability

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