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Updated: May 8, 2026

Stromal Cell Isolation From Hematopoietic Organs

Stromal Cell Isolation From Hematopoietic Organs

Published on: January 26, 2024

Primary marrow-derived stromal cells: isolation and manipulation.

Aravind Ramakrishnan¹, Beverly Torok-Storb, Manoj M Pillai

¹Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|August 21, 2013

Summary

Marrow stromal cells (MSCs) are rare but can be cultured from bone marrow. This chapter details methods to isolate, characterize, and manipulate these cells, including those from discarded bone marrow harvests.

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Area of Science:

Cell Biology
Stem Cell Research
Hematology

Background:

Marrow stromal cells (MSCs) are rare and difficult to detect in bone marrow samples.
While MSC populations can be expanded in vitro and differentiate into mesenchymal lineages, they are heterogeneous and not exclusively multipotent stem cells.
MSCs do not produce hematopoietic cells, distinguishing them from hematopoietic stem cells.

Purpose of the Study:

To describe methodology for deriving primary MSCs from bone marrow screens, a byproduct of clinical transplantation.
To present techniques for characterizing and manipulating both primary and immortalized MSCs.
To address the cellular heterogeneity within MSC cultures and donor variability.

Main Methods:

Isolation of primary MSCs from bone marrow mononuclear cells.

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Last Updated: May 8, 2026

Stromal Cell Isolation From Hematopoietic Organs

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Published on: January 26, 2024

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

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Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells

Published on: July 11, 2025

Utilizing bone marrow screens, a discarded byproduct of bone marrow harvests, as a source for MSCs.

Employing various techniques for characterization and manipulation of MSCs, including immortalized cell lines.

Main Results:

Established protocols for deriving primary MSCs from bone marrow screens.
Demonstrated methods for characterizing MSC populations, acknowledging their heterogeneity.
Provided techniques for manipulating MSCs for research and potential clinical applications.

Conclusions:

Bone marrow screens offer a viable source for obtaining primary MSCs.
Understanding MSC heterogeneity is crucial for their effective use in research and therapy.
Standardized methods for MSC characterization and manipulation are essential for consistent results.