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Updated: May 8, 2026

Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
Published on: September 26, 2010
Isolation and propagation of colon cancer stem cells
Pramudita R Prasetyanti1, Cheryl Zimberlin, Felipe De Sousa E Melo
1Laboratory of Experimental Oncology and Radiobiology Center for Experimental Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands.
Abstract:
The design of tissue culture conditions that faithfully reproduce the characteristics of cells in their native environment remains one of the main challenges of cancer stem cell (CSC) biology. Here we describe a detailed methodology for the isolation and expansion of both human colon CSCs and mouse intestinal adenoma together with a brief differentiation and coculture method that proved to be valuable to study the concept of CSCs plasticity.
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