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Updated: May 8, 2026

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
09:57

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Published on: June 14, 2020

Separation of biological proteins by liquid chromatography

Imran Ali1, Hassan Y Aboul-Enein, Prashant Singh

  • 1Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi 110 025, India.

Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society
|August 21, 2013
PubMed

Keywords:
2D-nano LC, two-dimensional nano liquid chromatography quadrupoleACN, acetonitrileAIEC, anion exchange chromatographyCEC, capillary electro-chromatographyCIEF, capillary isoelectric focusingCSF, cerebrospinal fluidChiralityEC, electro-chromatographyESI-LC–MS, electrospray ionization liquid chromatography–mass spectrometryFA, formic acidFLP, FMRF amide-like peptideFT-ICR-MS, ion cyclotron resonance-mass spectrometryGPI-APs, glycosylphosphadylinositol anchored proteinsGSH, glutathione stimulating hormoneGSTs, glutathione-S-transferase isoenzymeGeneHFBA, heptafluorobutyric acidHPLC, high performance liquid chromatographyICAT, isotope coded affinity tagIEF-SEC, isoelectrofocussing size-exclusion chromatographyIMCD, inner medullary collecting ductLC-Q-TOF, liquid chromatography-quadrupole time-of-flight tandem massLC-dual ESI, liquid chromatography dual electrospray ionization-Fourier transformLC–MS, liquid chromatography–mass spectrometryLiquid chromatographyMALDI-TOF, matrix-assisted laser desorption/ionization-time-of flightMFGM, milk fat globule membranesMMA, mass measurement accuracyMPC, mesenchymal progenitor cellMS/MS, spectrometryNLFs, Nasal lavage fluidsNLP, neuropeptide like proteinNano detectionPC2, prohormone convertase-2PS II, photosystem IIPreparationProteomicsQ-TOFMS/MS, time-of-flight tandem-mass spectrometryRPLC, reversed phase liquid chromatographySCX, strong cation exchangeSEC, size-exclusion chromatographyTFA, trifluoroacetic acidTIC, total ion currentTRAF, tumor necrosis factor receptor

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