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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Oogenesis02:07

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
Oogenesis01:22

Oogenesis

Oogenesis,  the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is known...

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Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro
09:47

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Published on: July 16, 2013

Pluripotent stem cells from maturing oocytes.

Alena Langerova1, Helena Fulka, Josef Fulka

  • 11 GENNET , Prague, Czech Republic .

Cellular Reprogramming
|August 22, 2013
PubMed
Summary
This summary is machine-generated.

This study explores inducing embryonic stem cell derivation from oocytes activated during maturation. We investigate if these transformed oocytes can develop into blastocysts for pluripotent stem cell lines.

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Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
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Human Egg Maturity Assessment and Its Clinical Application
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Human Egg Maturity Assessment and Its Clinical Application

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Human Egg Maturity Assessment and Its Clinical Application
08:51

Human Egg Maturity Assessment and Its Clinical Application

Published on: August 19, 2019

Area of Science:

  • Reproductive biology
  • Developmental biology
  • Stem cell science

Background:

  • Embryonic stem cells (ESCs) are typically derived from fertilized or parthenogenetically activated oocytes that reach the blastocyst stage.
  • Fertilization or activation triggers oocyte meiotic exit, chromosome decondensation, and mitotic entry, essential for development.
  • Oocyte chromatin decondensation and nuclear formation can be induced at various meiotic maturation stages post-germinal vesicle breakdown.

Purpose of the Study:

  • To investigate the potential for inducing cleavage and blastocyst development in oocytes experimentally manipulated during maturation.
  • To explore the feasibility of deriving pluripotent stem cell lines from such developmentally competent, transformed oocytes.

Main Methods:

  • Experimental manipulation of oocyte maturation stages.
  • Induction of chromatin decondensation and nuclear formation.
  • Assessment of oocyte cleavage and blastocyst formation potential.

Main Results:

  • Discussion of the theoretical possibility of inducing developmental progression in oocytes at different meiotic stages.
  • Exploration of the conditions required for achieving blastocyst development from transformed oocytes.

Conclusions:

  • Transformed maturing oocytes may possess the potential for cleavage and blastocyst development.
  • Successful derivation of pluripotent stem cell lines from these oocytes is a plausible, albeit challenging, prospect.