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Related Experiment Video

Updated: May 8, 2026

Gavaging Adult Zebrafish
03:09

Gavaging Adult Zebrafish

Published on: August 11, 2013

Gavaging adult zebrafish.

Chereen Collymore1, Skye Rasmussen, Ravi J Tolwani

  • 1Tri-Institutional Training Program in Laboratory Animal Medicine and Science Memorial Sloan-Kettering Cancer Center, Weill Cornell Medical College, The Rockefeller University, USA. ccollymore@rockefeller.edu

Journal of Visualized Experiments : Jove
|August 22, 2013
PubMed
Summary

A new gavage procedure precisely delivers compounds to adult zebrafish, overcoming limitations of oral administration for biomedical research. This method ensures accurate dosing for infectious disease and compound studies in this vital in vivo model.

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Area of Science:

  • * Zebrafish as a model organism in biomedical research.
  • * Development of novel drug delivery systems for aquatic models.

Background:

  • * Accurate compound administration is crucial for in vivo research.
  • * Current oral administration methods in adult zebrafish lack precision due to variable fish consumption.
  • * Need for reliable methods to deliver agents for scientific studies in zebrafish.

Purpose of the Study:

  • * To develop and validate a precise gavage procedure for adult zebrafish.
  • * To enable accurate delivery of compounds and infectious agents.
  • * To establish an efficient method for administering solutions to zebrafish.

Main Methods:

  • * Anesthesia of adult zebrafish (over 6 months) using buffered MS-222 (150 mg/L).
  • * Gavage administration of 5 μl solution via flexible catheter tubing attached to a 22-G needle.

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Last Updated: May 8, 2026

Gavaging Adult Zebrafish
03:09

Gavaging Adult Zebrafish

Published on: August 11, 2013

Microgavage of Zebrafish Larvae
10:53

Microgavage of Zebrafish Larvae

Published on: February 20, 2013

Partial Hepatectomy in Adult Zebrafish
06:06

Partial Hepatectomy in Adult Zebrafish

Published on: April 4, 2021

  • * Insertion of tubing into the oral cavity, past the gills, with slow injection into the intestinal tract.

    • Main Results:

    • * The gavage procedure demonstrated an 88% effectiveness rate.
    • * Zebrafish recovered uneventfully from the procedure.
    • * The method is efficient, allowing one person to process 20-30 fish per hour.

    Conclusions:

    • * The developed gavage technique provides a precise and effective means of compound delivery in adult zebrafish.
    • * This method overcomes the inaccuracies associated with voluntary oral consumption.
    • * Applicable for infectious disease studies and research involving precise administration of various compounds in zebrafish.