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Related Experiment Video

Updated: May 8, 2026

A Sensitive Method to Quantify Senescent Cancer Cells
09:18

A Sensitive Method to Quantify Senescent Cancer Cells

Published on: August 2, 2013

A sensitive method to quantify senescent cancer cells.

Julie Cahu1, Brigitte Sola

  • 1MILPAT (EA 4652), Université de Caen Basse-Normandie. julie.cahu@unicaen.fr

Journal of Visualized Experiments : Jove
|August 22, 2013
PubMed
Summary

Senescence, a cell cycle arrest, can be induced in cancer cells by chemotherapy. A new rapid, sensitive flow cytometry assay for studying senescence in live cancer cells is proposed, overcoming limitations of the standard β-galactosidase assay.

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Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry
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Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

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Last Updated: May 8, 2026

A Sensitive Method to Quantify Senescent Cancer Cells
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Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
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Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry
14:01

Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

Published on: September 13, 2022

Area of Science:

  • Cell Biology
  • Cancer Research
  • Biotechnology

Background:

  • Cellular senescence is a stable cell cycle arrest triggered by various intrinsic and extrinsic cues, including telomere shortening and oncogene activation.
  • Senescence occurs in both normal and cancer cells, with chemotherapeutics known to induce it in malignant cells.
  • The role of senescence in tumorigenesis remains controversial, highlighting the need for precise methods to assess it, especially in patient-specific contexts.

Purpose of the Study:

  • To develop and validate a novel, rapid, and sensitive assay for detecting and analyzing cellular senescence.
  • To overcome the limitations of the traditional β-galactosidase assay for senescence detection.
  • To provide a method for studying senescence on live cells, enabling further characterization with additional markers.

Main Methods:

  • Development of a flow cytometry-based assay for senescence detection.
  • Utilizing live-cell analysis to assess senescence.
  • Coupling the senescence assay with immunolabeling for multiparametric analysis.

Main Results:

  • The proposed flow cytometry assay is rapid and sensitive for detecting senescence.
  • The assay allows for the analysis of live cells, preserving cellular integrity.
  • The method can be readily combined with immunolabeling techniques to study senescence in conjunction with other cellular markers.

Conclusions:

  • A novel flow cytometry assay offers a significant improvement over the standard β-galactosidase assay for studying cellular senescence.
  • This new method provides a rapid, sensitive, and versatile tool for senescence research, particularly in cancer biology.
  • The ability to analyze live cells and combine with immunolabeling opens new avenues for understanding senescence mechanisms and implications in disease.