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Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
Published on: May 2, 2019
Detection and genetic characterization of feline kobuviruses
Joon-Yee Chung1, Seong-Hee Kim, Yeon-Hee Kim
1Viral Disease Diagnostic Laboratory, Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, 480 Anyang-6-Dong, Anyang, 430-824, Republic of Korea.
Abstract:
In order to survey for feline kobuviruses infection, fecal samples (n = 39) of cats with diarrhea were collected during 2011-2012. Six (14.5%) of the fecal samples tested were positive for feline kobuviruses. The partial nucleotide sequences of feline kobuviruses based on the RNA-dependent RNA polymerase gene were compared to those of other species. Feline kobuviruses were most closely related to canine kobuvirus in terms of their amino acid and nucleotide levels. In a phylogenetic tree, feline kobuviruses were also closely clustered with canine kobuvirus, Aichi virus (human), and mouse kobuvirus. This is the first report of the detection and genetic characterization of feline kobuviruses.

