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Antigen identification using SEREX

Ugur Sahin1, Ozlem Türeci

  • 1Translational Oncology (TRON), Mainz, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|August 22, 2013
PubMed

Serological analysis of recombinant cDNA expression libraries (SEREX) allows systematic identification of antigens recognized by the spontaneous autoantibody repertoire of patients with cancer and autoimmune disease. SEREX is based on screening of lambda phage expression libraries constructed from diseased tissue with the autologous patient's serum. This chapter provides the key protocols for SEREX immunoscreening and for downstream validation of autoantigens.

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