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Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
Published on: October 3, 2018
Antigen discovery using whole-genome phage display libraries.
Elisa Beghetto1, Nicola Gargano
1Sigma-Tau Industrie Farmaceutiche Riunite SpA, Rome, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|August 22, 2013
Summary
This study constructed a whole-genome library of Streptococcus pneumoniae using lambda-display technology. This tool aids in understanding the human B-cell response to bacterial infection and colonization.
Area of Science:
- Biotechnology
- Immunology
- Microbiology
Background:
- Phage display technology has advanced biological process investigation and molecule isolation over the past 20 years.
- Lambda phage systems are utilized for creating display libraries of peptides and protein domains.
- Large cDNA and whole-genome libraries from pathogens are increasingly used for antigen discovery.
Purpose of the Study:
- To construct a whole-genome lambda-display library of Streptococcus pneumoniae.
- To utilize this library for dissecting the human B-cell response to S. pneumoniae infection and colonization.
Main Methods:
- Construction of a whole-genome lambda-display library.
- Application of the library for molecular dissection of host immune responses.
Main Results:
- Successfully generated a whole-genome library of Streptococcus pneumoniae.
- The library facilitates the study of B-cell responses against this common pathogen.
Conclusions:
- The developed whole-genome lambda-display library is a valuable tool for studying host-pathogen interactions.
- This approach enables detailed molecular analysis of the human immune response to Streptococcus pneumoniae.
