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Updated: May 8, 2026
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
Published on: January 30, 2019
High throughput detection of human neutrophil peptides from serum, saliva, and tear by anthrax lethal factor-modified
Yuanyuan Zhao1, Xu Zhang, Xiangdong Xue
1CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology , 11 Beiyitiao, Zhongguancun, Beijing 100190, China.
Abstract:
Human α defensins human neutrophil peptide 1-3 (HNP 1-3) are potential prognostic cancer biomarkers. Metalloprotein anthrax lethal factor (ALF) binds to HNP 1-3 in a Zn2+-dependent manner. We conjugated ALF to the surface of magnetic nanoparticles (MNP) to magnetically isolate the HNPs, and used Zn2+ to control the capture and release HNPs.
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