Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The Two-State Receptor Model01:29

The Two-State Receptor Model

The two-state receptor model explains a drug's interaction with receptors, such as G protein-coupled receptors and ligand-gated ion channels, to induce or inhibit a biological response. When no natural ligands are present, a receptor exists in an equilibrium of inactive (Ri) and active (Ra) conformations. The inactive form does not produce a response, while the active form generates a basal effect known as constitutive activity.
The binding affinity of a drug determines its interaction with one...
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Crossing Over01:30

Crossing Over

Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...
Quantitative Aspects of Drug-Receptor Interaction01:30

Quantitative Aspects of Drug-Receptor Interaction

The receptor occupancy theory connects a drug's response to the number of occupied receptors. With higher drug concentrations, more receptors are occupied, leading to increased responses. The formation of drug-receptor complexes involves association and dissociation rates, which reach equilibrium when the forward and backward reactions are equal. The equilibrium association constant (Ka) and its inverse, the equilibrium dissociation constant (Kd), indicate drug affinity. Higher Ka and lower Kd...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The MOlecular-Scale Biophysics Research Infrastructure (MOSBRI) Project and its Outcomes.

European biophysics journal : EBJ·2026
Same author

Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

First Clinical Report on Efficacy of Alternative European Viper Antivenoms in Treatment of <i>Vipera ammodytes</i> Envenomation in Croatia.

Toxins·2026
Same author

Brolucizumab in the Treatment of Proliferative Diabetic Retinopathy: The CONDOR Randomized Clinical Trial.

JAMA ophthalmology·2026
Same author

Correction: The cytotoxicity of gomesin peptides is mediated by the glycosphingolipid pathway and lipid-cholesterol interactions.

Cell death discovery·2026
Same author

Lactoferrin and Its Enzymatic Hydrolysates as Natural Antimicrobial and Antioxidant Agents for Food Preservation.

Foods (Basel, Switzerland)·2026
Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026
Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026
Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026
Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: May 8, 2026

Single-Copy Gene Locus Chromatin Purification in Saccharomyces cerevisiae
10:33

Single-Copy Gene Locus Chromatin Purification in Saccharomyces cerevisiae

Published on: November 17, 2023

Structural insight into LexA-RecA* interaction.

Lidija Kovačič1, Nejc Paulič, Adrijana Leonardi

  • 1Department of Molecular and Biomedical Sciences, JoŽef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia, Department of Biology, University of Ljubljana, Biotechnical Faculty, Večna pot 111, 1000 Ljubljana, Slovenia, National Institute of Chemistry, 1000 Ljubljana, Slovenia, Department of Chemistry and Biochemistry, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, SI-1000 Ljubljana, Slovenia and Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Jamova 39, 1000 Ljubljana, Slovenia.

Nucleic Acids Research
|August 23, 2013
PubMed
Summary
This summary is machine-generated.

Blocking the interaction between RecA protein and LexA repressor could reduce bacterial antibiotic resistance. Researchers modeled this complex, revealing key interaction sites to guide inhibitor design.

More Related Videos

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

Related Experiment Videos

Last Updated: May 8, 2026

Single-Copy Gene Locus Chromatin Purification in Saccharomyces cerevisiae
10:33

Single-Copy Gene Locus Chromatin Purification in Saccharomyces cerevisiae

Published on: November 17, 2023

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

Area of Science:

  • Bacteriology
  • Molecular Biology
  • Genetics

Background:

  • RecA protein is crucial for bacterial DNA repair and antibiotic resistance.
  • RecA interacts with LexA, a transcriptional repressor, to regulate the SOS response.
  • Targeting the RecA-LexA interaction offers a novel strategy to combat antibiotic resistance.

Purpose of the Study:

  • To map the interaction site between LexA and the active RecA-ssDNA filament (RecA*).
  • To generate a 3D model of the LexA-RecA* complex.
  • To guide the engineering of inhibitors that block RecA-LexA complex formation.

Main Methods:

  • Protein interaction mapping
  • Three-dimensional structural modeling
  • Experimental validation of model predictions

Main Results:

  • A detailed 3D model of the LexA-RecA* complex was generated.
  • LexA's N-terminal DNA-binding domain and C-terminal catalytic domain interact with RecA*.
  • Operator DNA binding by LexA was shown to sterically hinder RecA* interaction.

Conclusions:

  • The validated model provides critical insights into the bacterial DNA damage response.
  • Understanding the LexA-RecA* interaction is key to developing new strategies against antibiotic resistance.
  • This research paves the way for novel therapeutic interventions targeting bacterial survival mechanisms.